The positive significance of government-organized nationwide sports events in the post-epidemic era: The impact on citizens' psychological well-being, mindfulness level, and social avoidance distress / La importancia positiva de los eventos deportivos nacionales organizados por el gobierno en la era post-epidemia: el impacto en el bienestar psicológico, el nivel de atención plena y la angustia por evitación social de los ciudadanos

Based on the nationwide sports event "Health Walk" held in Ji'an City, we divided the subjects into sports participation group and non-sports participation group to investigate the differences between the two groups in social avoidance and distress, psychological well-being, and mindfulness level. We also examined the mediating role of mindfulness and social avoidance and distress in whether to participate in sports activities and psychological well-being. In addition, we investigated citizens' approval of the government and their city, and collected basic information about the subjects. We used a 2 (sports participation, non-sports participation) * 2 (high social avoidance and distress, low social avoidance and distress) * 2 (high mindfulness level, low mindfulness level) mixed design. We measured the subjects using Watson's Social Avoidance and Distress Scale, the short version of Ryff's Psychological Well-Being Questionnaire, the Chinese Short Version of the Five-Dimensional Mindfulness Scale, and general survey questionnaires. SPSS 27.0 and the PROCESS plug-in were used to process and analyze the data through descriptive statistics, independent sample t-tests, correlation analysis and mediation effect tests. The results showed that there were significant differences in social avoidance, distress, and mindfulness levels in whether to participate in sports activities (ps <0.001). Whether to participate in sports activities and psychological well-being were significantly correlated with mindfulness (t = 0.368, p <0.001; t = 0.250, p <0.01), and were significantly correlated with social avoidance and distress (t = -0.307, p <0.001; t = -0.443, p <0.001). Mindfulness and social avoidance fully mediated the influence of sports participation on psychological well-being (ps <0.001).(AU)

A Mixture of Baicalein, Wogonin, and Oroxylin-A Inhibits EMT in the A549 Cell Line via the PI3K/AKT-TWIST1-Glycolysis Pathway.

Non-small cell lung cancer (NSCLC) is a worldwide disease with a high morbidity and mortality rate, which is most derived from its metastasis. Some studies show that the epithelial-mesenchymal transition (EMT) process promotes lung cancer cell migration and invasion, leading to NSCLC metastasis. Total flavonoid aglycones extract (TFAE) isolated from Scutellaria baicalensis was reported to inhibit tumor growth and induce apoptosis. In this study, we found that baicalein, wogonin, and oroxylin-A were the active compounds of TFAE. After reconstructing with these three compounds [baicalein (65.8%), wogonin (21.2%), and oroxylin-A (13.0%)], the reconstructed TFAE (reTFAE) inhibited the EMT process of A549 cells. Then, bioinformatic technology was employed to elucidate the potential pharmacodynamic mechanism network of reTFAE. We identified the relationship between reTFAE and PI3K/Akt signaling pathways, with TWIST1 as the key protein. LY294002, the inhibitor of the PI3K/Akt signaling pathway, and knock-down TWIST1 could significantly enhance the efficacy of reTFAE, with increasing expression of epithelial markers and decreasing expression of mesenchymal markers in A549 cells at the same time. Furthermore, stable isotope dimethyl-labeled proteomics technology was conducted to complement the follow-up mechanism that the EMT-inhibition process may be realized through the glycolysis pathway. In conclusion, we claim that TWIST1-targeted flavonoids could provide a new strategy to inhibit EMT progress for the treatment of NSCLC.